Characterization of nuclear import and export signals determining the subcellular localization of WD repeat-containing protein 42A (WDR42A).

WD repeat-containing protein 42A (WDR42A) is a member of the WD40-repeat proteins. Here, we investigated the localization pattern of WDR42A in living cells. By mutational analysis, a nuclear localization signal, 114PRRRVQRKR122, was for the first time determined. The dominant negative, co-immunoprecipitation and GST pull-down results further demonstrated that the nuclear ...
import of WDR42A was mediated by karyopherin-α1/β1 in conjunction with the GTPase Ran. Additionally, a nuclear export signal, 39IEVEASDLSLSL50, was verified to be a functional NES, which mediated the nuclear export through Chromosome Region Maintenance 1 dependent pathway. All these data suggest WDR42A is a nucleocytoplasmic shuttling protein.
Mesh Terms:
Active Transport, Cell Nucleus, Animals, COS Cells, Carrier Proteins, Cell Nucleus, Cells, Cultured, Cercopithecus aethiops, HEK293 Cells, Humans, Immunoprecipitation, Nuclear Localization Signals, Transfection
FEBS Lett.
Date: Apr. 24, 2012
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