Exo70 interacts with the Arp2/3 complex and regulates cell migration.
The exocyst is a multiprotein complex essential for tethering secretory vesicles to specific domains of the plasma membrane for exocytosis. Here, we report that the exocyst component Exo70 interacts with the Arp2/3 complex, a key regulator of actin polymerization. We further show that the exocyst-Arp2/3 interaction is regulated by epidermal ... growth factor (EGF) signalling. Inhibition of Exo70 by RNA interference (RNAi) or antibody microinjection blocks the formation of actin-based membrane protrusions and affects various aspects of cell motility. We propose that Exo70, in addition to functioning in exocytosis, also regulates actin at the leading edges of migrating cells, therefore coordinating cytoskeleton and membrane traffic during cell migration.
Mesh Terms:
Actin-Related Protein 2-3 Complex, Animals, Cell Movement, Epidermal Growth Factor, HeLa Cells, Humans, Membrane Proteins, Mice, NIH 3T3 Cells, Protein Binding, Rats, Vesicular Transport Proteins
Actin-Related Protein 2-3 Complex, Animals, Cell Movement, Epidermal Growth Factor, HeLa Cells, Humans, Membrane Proteins, Mice, NIH 3T3 Cells, Protein Binding, Rats, Vesicular Transport Proteins
Nat. Cell Biol.
Date: Dec. 01, 2006
PubMed ID: 17086175
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