NF-kappaB activator Act1 associates with IL-1/Toll pathway adaptor molecule TRAF6.
NF-kappaB activator 1 (Act1), also called CIKS, is a recently identified protein with NF-kappaB and AP-1 activation activities through its association with the IkappaB kinase complex. We identified and confirmed that Act1 interacts with tumor necrosis factor receptor-associated factor 6 (TRAF6); notably, Act1 binds to TRAF6 only among TRAF family ... proteins. The amino-terminal half of Act1 is required for its interaction with the TRAF domain. Act1-mediated NF-kappaB activation was inhibited by a dominant-negative mutant of TRAF6 in a dose-dependent manner, and IL-1-induced NF-kappaB activation was inhibited by a high level of Act1 expression. Our results suggest that Act1 is involved in IL-1/Toll-mediated signaling through TRAF6.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Carrier Proteins, Cell Line, Drosophila Proteins, Humans, Interleukin-1, Membrane Glycoproteins, Models, Biological, Mutation, NF-kappa B, Protein Structure, Tertiary, Proteins, Receptors, Cell Surface, Signal Transduction, TNF Receptor-Associated Factor 6, Toll-Like Receptors, Tumor Necrosis Factor Receptor-Associated Peptides and Proteins, Two-Hybrid System Techniques
Adaptor Proteins, Signal Transducing, Carrier Proteins, Cell Line, Drosophila Proteins, Humans, Interleukin-1, Membrane Glycoproteins, Models, Biological, Mutation, NF-kappa B, Protein Structure, Tertiary, Proteins, Receptors, Cell Surface, Signal Transduction, TNF Receptor-Associated Factor 6, Toll-Like Receptors, Tumor Necrosis Factor Receptor-Associated Peptides and Proteins, Two-Hybrid System Techniques
FEBS Lett.
Date: Dec. 04, 2002
PubMed ID: 12459498
View in: Pubmed Google Scholar
Download Curated Data For This Publication
145512
Switch View:
- Interactions 3