Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage-gated K(+) channel Kv2.1.
We report identification and characterization of Kv6.3, a novel member of the voltage-gated K(+) channel. Reverse transcriptase-polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage-gated ion channel. When Kv6.3 and Kv2.1 were co-expressed, the ... heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co-transfected cells. These results indicate that Kv6.3 is a novel member of the voltage-gated K(+) channel which functions as a modulatory subunit.
Mesh Terms:
Amino Acid Sequence, Cloning, Molecular, Delayed Rectifier Potassium Channels, Electric Conductivity, Humans, Ion Channel Gating, Molecular Sequence Data, Potassium Channels, Potassium Channels, Voltage-Gated, Protein Subunits, Sequence Homology, Amino Acid, Shab Potassium Channels, Tissue Distribution
Amino Acid Sequence, Cloning, Molecular, Delayed Rectifier Potassium Channels, Electric Conductivity, Humans, Ion Channel Gating, Molecular Sequence Data, Potassium Channels, Potassium Channels, Voltage-Gated, Protein Subunits, Sequence Homology, Amino Acid, Shab Potassium Channels, Tissue Distribution
FEBS Lett.
Date: Feb. 13, 2002
PubMed ID: 11852086
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