SOSS complexes participate in the maintenance of genomic stability.
Proteins that bind to single-stranded DNA (ssDNA) are essential for DNA replication, recombinational repair, and maintenance of genomic stability. Here, we describe the characterization of an ssDNA-binding heterotrimeric complex, SOSS (sensor of ssDNA) in human, which consists of human SSB homologs hSSB1/2 (SOSS-B1/2) and INTS3 (SOSS-A) and a previously uncharacterized ... protein C9orf80 (SOSS-C). We have shown that SOSS-A serves as a central adaptor required not only for SOSS complex assembly and stability, but also for facilitating the accumulation of SOSS complex to DNA ends. Moreover, SOSS-depleted cells display increased ionizing radiation sensitivity, defective G2/M checkpoint, and impaired homologous recombination repair. Thus, our study defines a pathway involving the sensing of ssDNA by SOSS complex and suggests that this SOSS complex is likely involved in the maintenance of genome stability.
Mesh Terms:
Carrier Proteins, Cell Cycle, DNA Breaks, Double-Stranded, DNA Repair, DNA, Single-Stranded, DNA-Binding Proteins, Genomic Instability, Humans, Nuclear Proteins, Protein Stability, Recombination, Genetic
Carrier Proteins, Cell Cycle, DNA Breaks, Double-Stranded, DNA Repair, DNA, Single-Stranded, DNA-Binding Proteins, Genomic Instability, Humans, Nuclear Proteins, Protein Stability, Recombination, Genetic
Mol. Cell
Date: Aug. 14, 2009
PubMed ID: 19683501
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