Yeast Ku as a regulator of chromosomal DNA end structure.
During telomere replication in yeast, chromosome ends acquire an S-phase-specific overhang of the guanosine-rich strand. Here it is shown that in cells lacking Ku, a heterodimeric protein involved in nonhomologous DNA end joining, these overhangs are present throughout the cell cycle. In vivo cross-linking experiments demonstrated that Ku is bound ... to telomeric DNA. These results show that Ku plays a direct role in establishing a normal DNA end structure on yeast chromosomes, conceivably by functioning as a terminus-binding factor. Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku has a distinct function when bound to telomeres.
Mesh Terms:
Antigens, Nuclear, Binding Sites, Chromosomes, Fungal, DNA Helicases, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, G2 Phase, Genes, Fungal, Mitosis, Mutation, Nuclear Proteins, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Telomerase, Telomere, Temperature, Transformation, Genetic
Antigens, Nuclear, Binding Sites, Chromosomes, Fungal, DNA Helicases, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, G2 Phase, Genes, Fungal, Mitosis, Mutation, Nuclear Proteins, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Telomerase, Telomere, Temperature, Transformation, Genetic
Science
Date: May. 01, 1998
PubMed ID: 9563951
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