Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain.

Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies. Citron kinase is a Ser/The kinase that we previously reported to ...
enhance human immunodeficiency virus type 1 (HIV-1) virion production. However, the relationship between Hrs and citron kinase in HIV-1 production remains elusive. Here, we report that Hrs interacts with citron kinase via its FYVE domain. Overexpression of Hrs or the FYVE domain resulted in a significant decrease in HIV-1 virion production. Depletion of Hrs by RNA interference in HEK293T cells increased HIV-1 virion production and enhanced the activity of citron kinase. These data suggest that Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis.
Mesh Terms:
Down-Regulation, Endosomal Sorting Complexes Required for Transport, Endosomes, Exocytosis, Gene Expression, Gene Silencing, HEK293 Cells, HIV Infections, HIV-1, Humans, Immunoprecipitation, Intracellular Signaling Peptides and Proteins, Microscopy, Fluorescence, Phosphoproteins, Plasmids, Protein Binding, Protein Interaction Domains and Motifs, Protein Structure, Tertiary, Protein Transport, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Transfection, Virion, Virus Release, Virus Replication
Protein Cell
Date: Jun. 01, 2011
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