Civ1 (CAK in vivo), a novel Cdk-activating kinase.
Cyclin-dependent protein kinases (Cdks) play key roles in regulating cell division and gene expression. Most Cdks require binding of a cyclin and phosphorylation by a Cdk-activating kinase (CAK) to be active. We report the identification of Civ1 (CAK in vivo), a novel CAK activity in S. cerevisiae. Civ1 is most ... similar in sequence to the Cdks, but unlike them is active as a monomer and may thus be the founding member of a novel family of kinases. Civ1 binds tightly to and phosphorylates Cdc28, thereby allowing its subsequent activation by the binding of a cyclin. The CIV1 gene is essential for yeast cell viability, and Cdc28 phosphorylation and activity are conditionally inhibited in a civ1-4 temperature-sensitive mutant. Civ1 is the only CAK for which there are genetic data indicating that its activity is physiologically relevant in vivo.
Mesh Terms:
Amino Acid Sequence, Base Sequence, CDC28 Protein Kinase, S cerevisiae, Cell Cycle, Cell Cycle Proteins, Cyclin-Dependent Kinases, DNA Primers, Fungal Proteins, Genes, Fungal, Molecular Sequence Data, Phosphorylation, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Sequence Alignment, Sequence Homology, Amino Acid
Amino Acid Sequence, Base Sequence, CDC28 Protein Kinase, S cerevisiae, Cell Cycle, Cell Cycle Proteins, Cyclin-Dependent Kinases, DNA Primers, Fungal Proteins, Genes, Fungal, Molecular Sequence Data, Phosphorylation, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Sequence Alignment, Sequence Homology, Amino Acid
Cell
Date: Aug. 23, 1996
PubMed ID: 8752211
View in: Pubmed Google Scholar
Download Curated Data For This Publication
14630
Switch View:
- Interactions 3
- PTM Genes 1