BIM1 encodes a microtubule-binding protein in yeast.
A previously uncharacterized yeast gene (YER016w) that we have named BIM1 (binding to microtubules) was obtained from a two-hybrid screen of a yeast cDNA library using as bait the entire coding sequence of TUB1 (encoding alpha-tubulin). Deletion of BIM1 results in a strong bilateral karyogamy defect, hypersensitivity to benomyl, and ... aberrant spindle behavior, all phenotypes associated with mutations affecting microtubules in yeast, and inviability at extreme temperatures (i.e., >/=37 degrees C or
Mesh Terms:
Alleles, Amino Acid Sequence, Cell Cycle, Cell Cycle Proteins, Cell Nucleus, Cell Size, Cytoskeleton, Fungal Proteins, Genes, Fungal, Genes, Lethal, Humans, Microtubule Proteins, Microtubule-Associated Proteins, Microtubules, Mitotic Spindle Apparatus, Molecular Sequence Data, Mutation, Nuclear Proteins, Phenotype, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Tubulin, Two-Hybrid System Techniques
Alleles, Amino Acid Sequence, Cell Cycle, Cell Cycle Proteins, Cell Nucleus, Cell Size, Cytoskeleton, Fungal Proteins, Genes, Fungal, Genes, Lethal, Humans, Microtubule Proteins, Microtubule-Associated Proteins, Microtubules, Mitotic Spindle Apparatus, Molecular Sequence Data, Mutation, Nuclear Proteins, Phenotype, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Tubulin, Two-Hybrid System Techniques
Mol. Biol. Cell
Date: Dec. 01, 1997
PubMed ID: 9398684
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