An extragenic suppressor of prp24-1 defines genetic interaction between PRP24 and PRP21 gene products of Saccharomyces cerevisiae.
The temperature-sensitive prp24-1 mutation defines a gene product required for the first step in pre-mRNA splicing. PRP24 is probably a component of the U6 snRNP particle. We have applied genetic reversion analysis to identify proteins that interact with PRP24. Spontaneous revertants of the temperature-sensitive (ts)prp24-1 phenotype were analyzed for those ... that are due to extragenic suppression. We then extended our analysis to screen for suppressors that confer a distinct conditional phenotype. We have identified a temperature-sensitive extragenic suppressor, which was shown by genetic complementation analysis to be allelic to prp21-1. This suppressor, prp21-2, accumulates pre-mRNA at the non-permissive temperature, a phenotype similar to that of prp21-1. prp21-2 completely suppresses the splicing defect and restores in vivo levels of the U6 snRNA in the prp24-1 strain. Genetic analysis of the suppressor showed that prp21-2 is not a bypass suppressor of prp24-1. The suppression of prp24-1 by prp21-2 is gene specific and also allele specific with respect to both the loci. Genetic interactions with other components of the pre-spliceosome have also been studied. Our results indicate an interaction between PRP21, a component of the U2 snRNP, and PRP24, a component of the U6 snRNP. These results substantiate other data showing U2-U6 snRNA interactions.
Mesh Terms:
Alleles, Blotting, Northern, Crosses, Genetic, Fungal Proteins, Gene Expression Regulation, Fungal, Genetic Complementation Test, Genotype, Meiosis, Mutation, Phenotype, Ploidies, RNA Precursors, RNA Splicing, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Spliceosomes, Suppression, Genetic
Alleles, Blotting, Northern, Crosses, Genetic, Fungal Proteins, Gene Expression Regulation, Fungal, Genetic Complementation Test, Genotype, Meiosis, Mutation, Phenotype, Ploidies, RNA Precursors, RNA Splicing, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Spliceosomes, Suppression, Genetic
Mol. Gen. Genet.
Date: Feb. 25, 1996
PubMed ID: 8602141
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