The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition.

In characterizing a series of yeast (Saccharomyces cerevisiae) mutants synthetic lethal with U1 RNA, we have identified a yeast gene (MUD2) with sequence similarity to the well-studied metazoan splicing factor U2AF65. The biochemical characterization indicates that the MUD2 gene product (MUD2P) contacts pre-mRNA directly and is a component of the ...
pre-mRNA-U1 snRNP complex (commitment complex) that forms during early spliceosome assembly in yeast extracts. Unlike U1 snRNP itself, the association of MUD2P with pre-mRNA is dependent on a proper yeast branchpoint sequence. Genetic experiments indicate that MUD2P affects U2 snRNP addition. Moreover, experiments in the two-hybrid system show that PRP11P, a recently identified component of U2 snRNP, can interact directly with MUD2P. The experiments identify a specific inter-snRNP protein-protein contact that occurs during spliceosome assembly and more generally support substantial functional similarity between U2AF65 and MUD2P.
Mesh Terms:
Amino Acid Sequence, Cloning, Molecular, Consensus Sequence, Fungal Proteins, Genes, Lethal, Hemagglutinin Glycoproteins, Influenza Virus, Hemagglutinins, Viral, Models, Genetic, Molecular Sequence Data, RNA Precursors, RNA Processing, Post-Transcriptional, RNA-Binding Proteins, Recombinant Fusion Proteins, Ribonucleoprotein, U1 Small Nuclear, Ribonucleoprotein, U2 Small Nuclear, Ribonucleoproteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Spliceosomes, Viral Envelope Proteins
Genes Dev.
Date: Apr. 01, 1994
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