Synergistic efficacy of LBH and alphaB-crystallin through inhibiting transcriptional activities of p53 and p21.
LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes alphaB-crystallin. Co-immunoprecipitation and GST pull-down ... assays showed that LBH interacts with alphaB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, alphaB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or alphaB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both alphaB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and alphaB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.
Mesh Terms:
Animals, COS Cells, Cercopithecus aethiops, Crystallins, Humans, Immunoprecipitation, Oncogene Protein p21(ras), Subcellular Fractions, Trans-Activators, Transcription, Genetic, Tumor Suppressor Protein p53, Two-Hybrid System Techniques
Animals, COS Cells, Cercopithecus aethiops, Crystallins, Humans, Immunoprecipitation, Oncogene Protein p21(ras), Subcellular Fractions, Trans-Activators, Transcription, Genetic, Tumor Suppressor Protein p53, Two-Hybrid System Techniques
BMB Rep
Date: Jun. 01, 2010
PubMed ID: 20587334
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