DJ-1 binds to mitochondrial complex I and maintains its activity.
Parkinson's disease (PD) is caused by neuronal cell death, and oxidative stress and mitochondrial dysfunction are thought to be responsible for onset of PD. DJ-1, a causative gene product of a familial form of Parkinson's disease, PARK7, plays roles in transcriptional regulation and anti-oxidative stress. The possible mitochondrial function of ... DJ-1 has been proposed, but its exact function remains unclear. In this study, we found that DJ-1 directly bound to NDUFA4 and ND1, nuclear and mitochondrial DNA-encoding subunits of mitochondrial complex I, respectively, and was colocalized with complex I and that complex I activity was reduced in DJ-1-knockdown NIH3T3 and HEK293 cells. These findings suggest that DJ-1 is an integral mitochondrial protein and that DJ-1 plays a role in maintenance of mitochondrial complex I activity.
Mesh Terms:
Animals, Electron Transport Complex I, Gene Knockdown Techniques, Humans, Intracellular Signaling Peptides and Proteins, Mice, Oncogene Proteins, Parkinson Disease, Protein Binding
Animals, Electron Transport Complex I, Gene Knockdown Techniques, Humans, Intracellular Signaling Peptides and Proteins, Mice, Oncogene Proteins, Parkinson Disease, Protein Binding
Biochem. Biophys. Res. Commun.
Date: Dec. 18, 2009
PubMed ID: 19822128
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