Mas37p, a novel receptor subunit for protein import into mitochondria.

By screening a collection of Saccharomyces cerevisiae mutants temperature sensitive for growth on a nonfermentable carbon source, we have isolated a gene (termed MAS37) which encodes a novel receptor for protein import into mitochondria. Mas37p is a 37-kD outer membrane protein with two putative membrane-spanning regions. Inactivation of the MAS37 ...
gene renders cells temperature-sensitive for respiration-driven growth, inhibits import of precursors into isolated mitochondria, and is synthetically lethal with a deletion of one of the genes encoding the import receptors Mas70p or Mas20p. Inactivation of Mas37p with specific antibodies inhibits import of different precursors to different extents; the precursor specificity of Mas37p resembles that of the previously described import receptor Mas70p. Mas70p and Mas37p form a 1:1 complex in detergent extracts of mitochondria and overexpression of one protein enhances that of the other. We suggest that the Mas37p/Mas70p heterodimer functions as a receptor for protein import into yeast mitochondria and that the mitochondrial receptor system consists of hetero-oligomeric subcomplexes with distinct binding activities, but overlapping precursor specificities.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Cloning, Molecular, Cytosol, Gene Expression, Genes, Fungal, Macromolecular Substances, Membrane Proteins, Mitochondria, Models, Structural, Molecular Sequence Data, Mutation, Protein Conformation, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sulfates, Sulfur Radioisotopes, Temperature
J. Cell Biol.
Date: Apr. 01, 1995
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