Calmodulin-related CML24 Interacts with ATG4b and Affects Autophagy Progression in Arabidopsis.

Plants encounter environmental stress challenges distinct from those of other eukaryotes because of their relative non-mobility. Therefore, plants may have evolved distinct regulatory aspects of conserved cellular functions. Plants, like other eukaryotes, share aspects of both calcium and calmodulin-based cellular signaling and the autophagic process of cellular renewal. Here, we ...
report a novel function for an Arabidopsis calmodulin-related protein, CML24, and insight into ATG4-regulated autophagy. CML24 interacts with ATG4b in yeast 2-hybrid, in vitro pull-down, and transient tobacco cell transformation assays. Mutants with missense mutations in CML24 have aberrant ATG4 activity patterns in in vitro extract assays, ATG8 accumulation levels, GFP-ATG8 decorated cellular structures, and darkness-induced starvation recovery. Together these results support the conclusion that CML24 affects autophagy progression through interactions with ATG4. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Plant J.
Date: Oct. 08, 2012
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