Activation of yeast protein kinase C by Rho1 GTPase.
We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of ... Pkc1.rho1 mutants are also impaired in pathway activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP-dependent manner. Recombinant Rho1 confers upon Pkc1 the ability to be stimulated by phosphatidylserine, indicating that Rho1 controls signal transmission through Pkc1.
Mesh Terms:
Cell Cycle Proteins, Enzyme Activation, GTP Phosphohydrolases, GTP-Binding Proteins, Guanosine Triphosphate, Models, Biological, Mutagenesis, Site-Directed, Protein Kinase C, Recombinant Fusion Proteins, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae, rho GTP-Binding Proteins
Cell Cycle Proteins, Enzyme Activation, GTP Phosphohydrolases, GTP-Binding Proteins, Guanosine Triphosphate, Models, Biological, Mutagenesis, Site-Directed, Protein Kinase C, Recombinant Fusion Proteins, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae, rho GTP-Binding Proteins
J. Biol. Chem.
Date: Apr. 19, 1996
PubMed ID: 8621575
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