The ATM-related cofactor Tra1 is a component of the purified SAGA complex.
The SAGA histone acetyltransferase/transcriptional adaptor complex is composed of multiple transcriptional regulators including Ada, Spt, and TAFII proteins. Here we identify an additional novel subunit of the complex, Tra1, an ATM/PI-3-kinase-related homolog of the human TRRAP cofactor, which is essential for c-Myc and E2F-mediated oncogenic transformation. Mass spectrometry, immunoblotting, and ... immunoprecipitation experiments confirm the stable association of this protein within SAGA. In addition, the Tra1 protein is a component of at least two other histone acetyltransferase protein complexes. These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter.
Mesh Terms:
Acetyltransferases, Amino Acid Sequence, Cell Cycle Proteins, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Fungal Proteins, Histone Acetyltransferases, Immunoblotting, Molecular Sequence Data, Multienzyme Complexes, Protein Kinases, Protein-Serine-Threonine Kinases, Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Tumor Suppressor Proteins
Acetyltransferases, Amino Acid Sequence, Cell Cycle Proteins, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Fungal Proteins, Histone Acetyltransferases, Immunoblotting, Molecular Sequence Data, Multienzyme Complexes, Protein Kinases, Protein-Serine-Threonine Kinases, Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Tumor Suppressor Proteins
Mol. Cell
Date: Dec. 01, 1998
PubMed ID: 9885573
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