Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression.
Several eukaryotic multiprotein complexes, including the Saccharomyces cerevisiae Snf/Swi complex, remodel chromatin for transcription. In contrast to the Snf/Swi proteins, Sfh1p, a new Snf5p paralog, is essential for viability. The evolutionarily conserved domain of Sfh1p is sufficient for normal function, and Sfh1p interacts functionally and physically with an essential Snf2p ... paralog in a novel nucleosome-restructuring complex called RSC (for remodels the structure of chromatin). A temperature-sensitive sfh1 allele arrests cells in the G2/M phase of the cell cycle, and the Sfh1 protein is specifically phosphorylated in the G1 phase. Together, these results demonstrate a link between chromatin remodeling and progression through the cell division cycle, providing genetic clues to possible targets for RSC function.
Mesh Terms:
Adenosine Triphosphatases, Adenosine Triphosphate, Amino Acid Sequence, Animals, Caenorhabditis elegans, Cell Cycle, Cell Cycle Proteins, Chromatin, Chromosomal Proteins, Non-Histone, Conserved Sequence, DNA Helicases, DNA-Binding Proteins, Drosophila melanogaster, Fungal Proteins, Humans, Molecular Sequence Data, Nuclear Proteins, Nucleosomes, Phenotype, Phosphorylation, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors
Adenosine Triphosphatases, Adenosine Triphosphate, Amino Acid Sequence, Animals, Caenorhabditis elegans, Cell Cycle, Cell Cycle Proteins, Chromatin, Chromosomal Proteins, Non-Histone, Conserved Sequence, DNA Helicases, DNA-Binding Proteins, Drosophila melanogaster, Fungal Proteins, Humans, Molecular Sequence Data, Nuclear Proteins, Nucleosomes, Phenotype, Phosphorylation, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors
Mol. Cell. Biol.
Date: Jun. 01, 1997
PubMed ID: 9154831
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