Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK.

KIN28, a member of the p34cdc2/CDC28 family of protein kinases, is identified as a subunit of yeast RNA polymerase transcription factor IIH (TFIIH) on the basis of sequence determination, immunological reactivity, and copurification. KIN28 is, moreover, one of three subunits of TFIIK, a subassembly of TFIIH with protein kinase activity ...
directed toward the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II. Itself a phosphoprotein, KIN28 interacts specifically with the two largest subunits of RNA polymerase II. Previous work of others points to two further associations: KIN28 interacts in vivo with the cyclin CCL1, and KIN28 and CCL1 are homologous to human MO15 and cyclin H, which form the cyclin-dependent kinase-activating kinase (CAK). We show that human CAK possesses the CTD kinase activity characteristic of TFIIH.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Blotting, Western, Cell-Free System, Cyclin-Dependent Kinases, Cyclins, Humans, Molecular Sequence Data, Phosphoric Monoester Hydrolases, Protein Binding, Protein Biosynthesis, Protein-Serine-Threonine Kinases, RNA Polymerase II, Recombinant Proteins, Saccharomyces cerevisiae Proteins, Sequence Analysis, Sequence Homology, Amino Acid, TATA-Binding Protein Associated Factors, Transcription Factor TFIID, Transcription Factor TFIIH, Transcription Factors, Transcription Factors, TFII
Cell
Date: Dec. 16, 1994
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