Interaction of HSP90 to N-WASP leads to activation and protection from proteasome-dependent degradation.

Neural Wiskott-Aldrich syndrome protein (N-WASP) regulates reorganization of the actin cytoskeleton through activation of the Arp2/3 complex. Here, we show that heat shock protein 90 (HSP90) regulates N-WASP-induced actin polymerization in cooperation with phosphorylation of N-WASP. HSP90 binds directly to N-WASP, but binding alone does not affect the rate of ...
N-WASP/Arp2/3 complex-induced in vitro actin polymerization. An Src family tyrosine kinase, v-Src, phosphorylates and activates N-WASP. HSP90 increases the phosphorylation of N-WASP by v-Src, leading to enhanced N-WASP-dependent actin polymerization. In addition, HSP90 protects phosphorylated and activated N-WASP from proteasome-dependent degradation, resulting in amplification of N-WASP-dependent actin polymerization. Association between HSP90 and N-WASP is increased in proportion to activation of N-WASP by phosphorylation. HSP90 is colocalized and associated with active N-WASP at podosomes in 3Y1/v-Src cells and at growing neurites in PC12 cells, whose actin structures are clearly inhibited by blocking the binding of HSP90 to N-WASP. These findings suggest that HSP90 induces efficient activation of N-WASP downstream of phosphorylation signal by Src family kinases and is critical for N-WASP-dependent podosome formation and neurite extension.
Mesh Terms:
Actin-Related Protein 2, Actin-Related Protein 3, Actins, Animals, Binding Sites, Cell Line, Cell Surface Extensions, Cytoskeletal Proteins, Enzyme Activation, HSP90 Heat-Shock Proteins, Humans, Nerve Tissue Proteins, Oncogene Protein pp60(v-src), Phosphorylation, Proteasome Endopeptidase Complex, Protein Structure, Tertiary, Rats, Recombinant Fusion Proteins, Wiskott-Aldrich Syndrome Protein, Neuronal, src-Family Kinases
EMBO J.
Date: Apr. 20, 2005
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