p14ARF interacts with N-Myc and inhibits its transcriptional activity.
In this study, we report that the human p14(ARF) associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line ... p14(ARF) over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14(ARF) are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14(ARF) protein is involved in binding to c-Myc and N-Myc proteins.
Mesh Terms:
Binding Sites, Cell Line, Cell Line, Tumor, Cell Nucleolus, Humans, Neuroblastoma, Peptide Fragments, Protein Binding, Protein Structure, Tertiary, Proto-Oncogene Proteins c-myc, Recombinant Proteins, Sequence Deletion, Transcription, Genetic, Transfection, Tumor Suppressor Protein p14ARF
Binding Sites, Cell Line, Cell Line, Tumor, Cell Nucleolus, Humans, Neuroblastoma, Peptide Fragments, Protein Binding, Protein Structure, Tertiary, Proto-Oncogene Proteins c-myc, Recombinant Proteins, Sequence Deletion, Transcription, Genetic, Transfection, Tumor Suppressor Protein p14ARF
FEBS Lett.
Date: Mar. 06, 2007
PubMed ID: 17289033
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