Molecular cloning and characterization of CRLM-2, a novel type I cytokine receptor preferentially expressed in hematopoietic cells.
A murine expressed sequence tag (EST) showing homology with erythropoietin receptor (EPOR) was identified in the EST database. Cloning of the full-length cDNA revealed a 359 amino acid novel type I cytokine receptor, designated cytokine receptor like molecule-2 (CRLM-2). While CRLM-2 lacks typical WSXWS motif, it has a significant homology ... with EPOR, IL-2 receptor beta and gamma, and IL-9 receptor alpha. The murine CRLM-2 gene is composed of 8 exons, and an alternative mRNA splicing generates a variant transcript encoding a soluble CRLM-2. CRLM-2 is preferentially expressed in hematopoietic cells, particularly in hematopoietic progenitors and myeloid cells. Furthermore, CRLM-2 is constitutively associated with JAK2, a well-known tyrosine kinase that transmits signals from cytokine receptors. These data strongly suggest that CRLM-2 is a novel cytokine receptor involved in the regulation of hematopoietic system.
Mesh Terms:
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers, DNA, Complementary, Gene Expression, Hematopoietic System, Immunoglobulins, Mice, Molecular Sequence Data, RNA, Messenger, Receptors, Cytokine, Sequence Homology, Amino Acid, Signal Transduction
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers, DNA, Complementary, Gene Expression, Hematopoietic System, Immunoglobulins, Mice, Molecular Sequence Data, RNA, Messenger, Receptors, Cytokine, Sequence Homology, Amino Acid, Signal Transduction
Biochem. Biophys. Res. Commun.
Date: May. 27, 2000
PubMed ID: 10872831
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