The human synMuv-like protein LIN-9 is required for transcription of G2/M genes and for entry into mitosis.
Regulated gene expression is critical for the proper timing of cell cycle transitions. Here we report that human LIN-9 has an important function in transcriptional regulation of G2/M genes. Depletion of LIN-9 by RNAi in human fibroblasts strongly impairs proliferation and delays progression from G2 to M. We identify a ... cluster of G2/M genes as direct targets of LIN-9. Activation of these genes is linked to an association between LIN-9 and B-MYB. Chromatin immunoprecipitation assays revealed binding of both LIN-9 and B-MYB to the promoters of G2/M regulated genes. Depletion of B-MYB recapitulated the biological outcome of LIN-9 knockdown, including impaired proliferation and reduced expression of G2/M genes. These data suggest a critical role for human LIN-9, together with B-MYB, in the activation of genes that are essential for progression into mitosis.
Mesh Terms:
Cell Cycle Proteins, Cell Division, DNA Damage, DNA-Binding Proteins, Fibroblasts, G2 Phase, Gene Expression Regulation, HeLa Cells, Humans, Mitosis, Multigene Family, Nuclear Proteins, Promoter Regions, Genetic, Protein Structure, Tertiary, RNA Interference, Trans-Activators, Transcription, Genetic, Tumor Suppressor Proteins
Cell Cycle Proteins, Cell Division, DNA Damage, DNA-Binding Proteins, Fibroblasts, G2 Phase, Gene Expression Regulation, HeLa Cells, Humans, Mitosis, Multigene Family, Nuclear Proteins, Promoter Regions, Genetic, Protein Structure, Tertiary, RNA Interference, Trans-Activators, Transcription, Genetic, Tumor Suppressor Proteins
EMBO J.
Date: Jan. 10, 2007
PubMed ID: 17159899
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