The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.
We identified a complex in S. cerevisiae, the "exosome," consisting of the five essential proteins Rrp4p, Rrp41p, Rrp42p, Rrp43p, and Rrp44p (Dis3p). Remarkably, four of these proteins are homologous to characterized bacterial 3'-->5' exoribonucleases; Rrp44p is homologous to RNase II, while Rrp41p, Rrp42p, and Rrp43p are related to RNase PH. ... Recombinant Rrp4p, Rrp44p, and Rrp41p are 3'-->5' exoribonucleases in vitro that have distributive, processive, and phosphorolytic activities, respectively. All components of the exosome are required for 3' processing of the 5.8S rRNA. Human Rrp4p is found in a comparably sized complex, and expression of the hRRP4 gene in yeast complements the rrp4-1 mutation. We conclude that the exosome constitutes a highly conserved eukaryotic RNA processing complex.
Mesh Terms:
Amino Acid Sequence, Exoribonucleases, Fungal Proteins, Genetic Complementation Test, Hela Cells, Humans, Molecular Sequence Data, Molecular Weight, Multienzyme Complexes, Mutation, RNA Processing, Post-Transcriptional, RNA, Ribosomal, 5.8S, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Amino Acid Sequence, Exoribonucleases, Fungal Proteins, Genetic Complementation Test, Hela Cells, Humans, Molecular Sequence Data, Molecular Weight, Multienzyme Complexes, Mutation, RNA Processing, Post-Transcriptional, RNA, Ribosomal, 5.8S, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cell
Date: Nov. 14, 1997
PubMed ID: 9390555
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