Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane.

The mitochondrial inner membrane contains two separate translocons: one required for the translocation of matrix-targeted proteins (the Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex). To identify new members of the Tim54p-Tim22p complex, we screened for high-copy suppressors of the ...
temperature-sensitive tim54-1 mutant. We identified a new gene, TIM18, that encodes an integral protein of the inner membrane. The following genetic and biochemical observations suggest that the Tim18 protein is part of the Tim54p-Tim22p complex in the inner membrane: multiple copies of TIM18 suppress the tim54-1 growth defect; the tim18::HIS3 disruption is synthetically lethal with tim54-1; Tim54p and Tim22p can be coimmune precipitated with the Tim18 protein; and Tim18p, along with Tim54p and Tim22p, is detected in an approximately 300-kDa complex after blue native electrophoresis. We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane.
Mesh Terms:
Amino Acid Sequence, Animals, Antiporters, Base Sequence, Binding Sites, Biological Transport, Carrier Proteins, Cell Division, Fungal Proteins, Hydro-Lyases, Intracellular Membranes, Membrane Proteins, Membrane Transport Proteins, Mitochondria, Mitochondrial Membrane Transport Proteins, Molecular Sequence Data, Rabbits, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Mol. Biol. Cell
Date: Jan. 01, 2000
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