Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane.
The mitochondrial inner membrane contains two separate translocons: one required for the translocation of matrix-targeted proteins (the Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex). To identify new members of the Tim54p-Tim22p complex, we screened for high-copy suppressors of the ... temperature-sensitive tim54-1 mutant. We identified a new gene, TIM18, that encodes an integral protein of the inner membrane. The following genetic and biochemical observations suggest that the Tim18 protein is part of the Tim54p-Tim22p complex in the inner membrane: multiple copies of TIM18 suppress the tim54-1 growth defect; the tim18::HIS3 disruption is synthetically lethal with tim54-1; Tim54p and Tim22p can be coimmune precipitated with the Tim18 protein; and Tim18p, along with Tim54p and Tim22p, is detected in an approximately 300-kDa complex after blue native electrophoresis. We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane.
Mesh Terms:
Amino Acid Sequence, Animals, Antiporters, Base Sequence, Binding Sites, Biological Transport, Carrier Proteins, Cell Division, Fungal Proteins, Hydro-Lyases, Intracellular Membranes, Membrane Proteins, Membrane Transport Proteins, Mitochondria, Mitochondrial Membrane Transport Proteins, Molecular Sequence Data, Rabbits, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Amino Acid Sequence, Animals, Antiporters, Base Sequence, Binding Sites, Biological Transport, Carrier Proteins, Cell Division, Fungal Proteins, Hydro-Lyases, Intracellular Membranes, Membrane Proteins, Membrane Transport Proteins, Mitochondria, Mitochondrial Membrane Transport Proteins, Molecular Sequence Data, Rabbits, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Mol. Biol. Cell
Date: Jan. 01, 2000
PubMed ID: 10637294
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