A high-throughput approach for measuring temporal changes in the interactome.

Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS ...
with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.
Mesh Terms:
Amino Acids, Cell Culture Techniques, Chromatography, Gel, Chromatography, High Pressure Liquid, High-Throughput Screening Assays, Humans, Isotope Labeling, Protein Interaction Mapping, Protein Interaction Maps, Proteins, Proteomics, Time Factors
Nat. Methods
Date: Sep. 01, 2012
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