A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae.
We describe a new minichromosome maintenance factor, Mcm10, and show that this essential protein is involved in the initiation of DNA replication in Saccharomyces cerevisiae. The mcm10 mutant has an autonomously replicating sequence-specific minichromosome maintenance defect and arrests at the nonpermissive temperature with dumbbell morphology and 2C DNA content. Mcm10 ... is a nuclear protein that physically interacts with several members of the MCM2-7 family of DNA replication initiation factors. Cloning and sequencing of the MCM10 gene show that it is identical to DNA43, a gene identified independently for its putative role in replicating DNA. Two-dimensional DNA gel analysis reveals that the mcm10-1 lesion causes a dramatic reduction in DNA replication initiation at chromosomal origins, including ORI1 and ORI121. Interestingly, the mcm10-1 lesion also causes replication forks to pause during elongation through these same loci. This novel phenotype suggests a unique role for the Mcm10 protein in the initiation of DNA synthesis at replication origins.
Mesh Terms:
Alleles, Cell Cycle, Cell Cycle Proteins, Cell Division, Cell Nucleus, Chromosomal Proteins, Non-Histone, Cloning, Molecular, DNA Replication, DNA, Fungal, Fungal Proteins, Nuclear Proteins, Nucleic Acid Conformation, Phenotype, Plasmids, Replication Origin, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature
Alleles, Cell Cycle, Cell Cycle Proteins, Cell Division, Cell Nucleus, Chromosomal Proteins, Non-Histone, Cloning, Molecular, DNA Replication, DNA, Fungal, Fungal Proteins, Nuclear Proteins, Nucleic Acid Conformation, Phenotype, Plasmids, Replication Origin, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature
Mol. Cell. Biol.
Date: Jun. 01, 1997
PubMed ID: 9154825
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