TORC1-regulated protein kinase Npr1 phosphorylates Orm to stimulate complex sphingolipid synthesis.
The evolutionarily conserved Orm1 and Orm2 proteins mediate sphingolipid homeostasis. However, the homologous Orm proteins and the signaling pathways modulating their phosphorylation and function are incompletely characterized. Here we demonstrate that inhibition of nutrient-sensitive target of rapamycin complex 1 (TORC1) stimulates Orm phosphorylation and synthesis of complex sphingolipids in Saccharomyces ... cerevisiae. TORC1 inhibition activates the kinase Npr1 which directly phosphorylates and activates the Orm proteins. Npr1-phosphorylated Orm1 and Orm2 stimulate de novo synthesis of complex sphingolipids downstream of serine palmitoyltransferase (SPT). Complex sphingolipids in turn stimulate plasma membrane localization and activity of the nutrient scavenging general amino acid permease Gap1. Thus, activation of Orm and complex sphingolipid synthesis upon TORC1 inhibition is a physiological response to starvation.
Mol. Biol. Cell
Date: Jan. 30, 2013
PubMed ID: 23363605
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