Proteomic analysis identifies that 14-3-3zeta interacts with beta-catenin and facilitates its activation by Akt.

beta-Catenin is a central effector of Wnt signaling in embryonic and stem cell development and in tumorigenesis. Here, through a mass spectrometric analysis of a beta-catenin protein complex, we identified 12 proteins as putative beta-catenin interactors. We show that one of them, 14-3-3zeta, enhances beta-catenin-dependent transcription by maintaining a high ...
level of beta-catenin protein in the cytoplasm. More importantly, 14-3-3zeta facilitates activation of beta-catenin by the survival kinase Akt and colocalizes with activated Akt in intestinal stem cells. We propose that Akt phosphorylates beta-catenin, which results in 14-3-3zeta binding and stabilization of beta-catenin, and these interactions may be involved in stem cell development.
Mesh Terms:
14-3-3 Proteins, Amino Acid Sequence, Animals, Cell Line, Cricetinae, Cytoskeletal Proteins, Humans, Molecular Sequence Data, Precipitin Tests, Promoter Regions, Genetic, Protein Binding, Protein-Serine-Threonine Kinases, Proteome, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-akt, Trans-Activators, beta Catenin
Proc. Natl. Acad. Sci. U.S.A.
Date: Oct. 26, 2004
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