Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription.
We have identified a conserved region in the C-terminal domain of bromodomain-containing protein 4 (BRD4) that mediates its specific interaction with positive transcription elongation factor b (P-TEFb). This domain is highly conserved in testis-specific bromodomain protein (BRDT) and Drosophila fs(1)h. Both BRDT and fs(1)h specifically interact with P-TEFb in mammalian ... cells, and this interaction depends on their C-terminal domains. Overexpression of the BRD4 P-TEFb-interacting domain disrupts the interaction between the HIV transactivator Tat and P-TEFb and suppresses the ability of Tat to transactivate the HIV promoter. Incubation of cells with a synthetic peptide containing the C-terminal domain of BRD4 interferes with transactivation of the HIV promoter by the Tat protein.
Mesh Terms:
Amino Acid Sequence, Animals, Cell Line, Conserved Sequence, Gene Products, tat, HIV, Humans, Molecular Sequence Data, Nuclear Proteins, Positive Transcriptional Elongation Factor B, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Sequence Alignment, Transcription Factors, Transcription, Genetic, Transcriptional Activation, tat Gene Products, Human Immunodeficiency Virus
Amino Acid Sequence, Animals, Cell Line, Conserved Sequence, Gene Products, tat, HIV, Humans, Molecular Sequence Data, Nuclear Proteins, Positive Transcriptional Elongation Factor B, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Sequence Alignment, Transcription Factors, Transcription, Genetic, Transcriptional Activation, tat Gene Products, Human Immunodeficiency Virus
Proc. Natl. Acad. Sci. U.S.A.
Date: Aug. 21, 2007
PubMed ID: 17690245
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