Proteomic analysis reveals novel binding partners of MIP-T3 in human cells.
MIP-T3 (microtubule-interacting protein associated with TRAF3) is a microtubule-interacting protein that evolutionarily conserved from worms to humans, but whose cellular functions remains unknown. To get insight into the functions of MIP-T3, we set out to identify MIP-T3 interacting proteins by immunoprecipitation in human embryonic kidney 293 cells and MS analysis. ... As the results, a total of 34 proteins were identified and most of them were novel MIP-T3 putative partners. The MIP-T3-associated proteins could be grouped into nine clusters based on their molecule functions, including cytoskeleton, chaperone, nucleic acid binding, kinase and so on. Three MIP-T3-interacted proteins - actin, HSPA8 and tubulin - were further confirmed by reciprocal coimmunoprecipitations and colocalization analysis. The interaction of MIP-T3 with both actin filaments and microtubule suggested that MIP-T3 may play an important role in regulation of cytoskeleton dynamics in cells. Our results therefore not only uncover a large number of MIP-T3-associated proteins that possess a variety of cellular functions, but also provide new research directions for the study of the functions of MIP-T3.
Mesh Terms:
Actins, Blotting, Western, Carrier Proteins, Cell Line, Cell Line, Tumor, Humans, Immunoprecipitation, Microscopy, Confocal, Microtubule-Associated Proteins, Microtubules, Protein Binding, Proteomics
Actins, Blotting, Western, Carrier Proteins, Cell Line, Cell Line, Tumor, Humans, Immunoprecipitation, Microscopy, Confocal, Microtubule-Associated Proteins, Microtubules, Protein Binding, Proteomics
Proteomics
Date: Jun. 01, 2010
PubMed ID: 20391533
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