Per3, a circadian gene, is required for Chk2 activation in human cells.
PER3 is a member of the PERIOD genes, but does not play essential roles in the circadian clock. Depletion of Per3 by siRNA almost completely abolished activation of checkpoint kinase 2 (Chk2) after inducing DNA damage in human cells. In addition, Per3 physically interacted with ATM and Chk2. Per3 overexpression ... induced Chk2 activation in the absence of exogenous DNA damage, and this activation depended on ATM. Per3 overexpression also led to the inhibition of cell proliferation and apoptotic cell death. These combined results suggest that Per3 is a checkpoint protein that plays important roles in checkpoint activation, cell proliferation and apoptosis.
Mesh Terms:
Apoptosis, Cell Cycle Proteins, Cell Proliferation, Circadian Clocks, DNA Damage, DNA-Binding Proteins, Down-Regulation, Enzyme Activation, Gene Knockdown Techniques, HeLa Cells, Humans, Period Circadian Proteins, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Signal Transduction, Tumor Suppressor Proteins
Apoptosis, Cell Cycle Proteins, Cell Proliferation, Circadian Clocks, DNA Damage, DNA-Binding Proteins, Down-Regulation, Enzyme Activation, Gene Knockdown Techniques, HeLa Cells, Humans, Period Circadian Proteins, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Signal Transduction, Tumor Suppressor Proteins
FEBS Lett.
Date: Dec. 01, 2010
PubMed ID: 21070773
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