Xue YM, Kowalska AK, Grabowska K, Przybyt K, Cichewicz MA, Del Rosario BC, Pemberton LF

Histone chaperones function in chromatin assembly and disassembly suggesting they have important regulatory roles in transcription elongation. The Saccharomyces cerevisiae proteins, Nap1 and Vps75, are structurally related, evolutionarily conserved histone chaperones. We showed that Nap1 genetically interacts with several transcription elongation factors, and both Nap1 and Vps75 interact with the ...

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RNA polymerase II kinase, CTK1. Loss of NAP1 or VPS75 suppressed cryptic transcription within the ORF observed when strains are deleted for the kinase CTK1. Loss of the histone acetyl-transferase Rtt109 also suppressed ctk1-dependent cryptic transcription. Vps75 regulates Rtt109 function, suggesting that they function together in this process. H3 K9 was found to be the important lysine that is acetylated by Rtt109 during ctk1-dependent cryptic transcription. We showed that both Vps75 and Nap1 regulate the relative level of H3 K9 acetylation in the STE11 ORF. This supports a model in which Nap1, like Vps75, directly regulates Rtt109 activity, or regulates the assembly of acetylated chromatin. Although Nap1 and Vps75 share many similarities, due to their distinct interactions with SET2, Nap1 and Vps75, may also play separate roles during transcription elongation. This work sheds further light on the importance of histone chaperones as general regulators of transcription elongation.

Date: Feb. 11, 2013

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