A protein kinase substrate identified by the two-hybrid system.
A genetic method, the two-hybrid system, was used to identify four genes encoding proteins that interact with the SNF1 protein kinase from yeast. One of the genes, SIP1, was independently isolated as a multicopy suppressor of defects caused by reduced SNF1 kinase activity, and genetic evidence supports its function in ... the SNF1 pathway. The SIP1 protein co-immunoprecipitated with SNF1 and was phosphorylated in vitro. Thus, the two-hybrid system, which is applicable to any cloned gene, can be used to detect physical interactions between protein kinases and functionally related substrate proteins.
Mesh Terms:
AMP-Activated Protein Kinases, Amino Acid Sequence, Base Sequence, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Molecular Sequence Data, Mutagenesis, Phosphorylation, Plasmids, Protein Kinases, Protein-Serine-Threonine Kinases, Recombinant Fusion Proteins, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Substrate Specificity, Transcription Factors
AMP-Activated Protein Kinases, Amino Acid Sequence, Base Sequence, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Molecular Sequence Data, Mutagenesis, Phosphorylation, Plasmids, Protein Kinases, Protein-Serine-Threonine Kinases, Recombinant Fusion Proteins, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Substrate Specificity, Transcription Factors
Science
Date: Jul. 31, 1992
PubMed ID: 1496382
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