SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2.

The yeast SNF-SWI complex is required for transcriptional activation of diverse genes and has been shown to alter chromatin structure. The complex has at least 10 components, including SNF2/SWI2, SNF5, SNF6, SWI1/ADR6, and SWI3, and has been widely conserved in eukaryotes. Here we report the characterization of a new component. ...
We identified proteins that interact in the two-hybrid system with the N-terminal region of SNF2, preceding the ATPase domain. In addition to SWI3, we recovered a new 19-kDa protein, designated SNF11. Like other SNF/SWI proteins, SNF11 functions as a transcriptional activator in genetic assays. SNF11 interacts with SNF2 in vitro and copurifies with the SNF-SWI complex from yeast cells. Using a specific antibody, we showed that SNF11 coimmunoprecipitates with members of the SNF-SWI complex and that SNF11 is tightly and stoichiometrically associated with the complex. Furthermore, SNF11 was detected in purified SNF-SWI complex by staining with Coomassie blue dye; its presence previously went unrecognized because it does not stain with silver. SNF11 interacts with a 40-residue sequence of SNF2 that is highly conserved, suggesting that SNF11 homologs exist in other organisms.
Mesh Terms:
Adenosine Triphosphatases, Amino Acid Sequence, Base Sequence, Conserved Sequence, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Reporter, Macromolecular Substances, Molecular Sequence Data, Mutagenesis, Nuclear Proteins, Precipitin Tests, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Selection, Genetic, Sequence Homology, Amino Acid, Trans-Activators, Transcription Factors
Mol. Cell. Biol.
Date: Aug. 01, 1995
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