Functional analysis of the subunits of the chromatin assembly factor RSF.
The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the ... largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.
Mesh Terms:
Adenosine Triphosphatases, Amino Acid Sequence, Chromatin, Chromosomal Proteins, Non-Histone, Cloning, Molecular, Gene Expression Regulation, HeLa Cells, Histones, Humans, Molecular Sequence Data, Molecular Weight, Nuclear Proteins, Protein Structure, Tertiary, Recombinant Proteins, Sequence Homology, Amino Acid, Trans-Activators
Adenosine Triphosphatases, Amino Acid Sequence, Chromatin, Chromosomal Proteins, Non-Histone, Cloning, Molecular, Gene Expression Regulation, HeLa Cells, Histones, Humans, Molecular Sequence Data, Molecular Weight, Nuclear Proteins, Protein Structure, Tertiary, Recombinant Proteins, Sequence Homology, Amino Acid, Trans-Activators
Mol. Cell. Biol.
Date: Oct. 01, 2003
PubMed ID: 12972596
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