CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly.

CWH41 encodes a novel type II integral membrane N-glycoprotein located in the endoplasmic reticulum. Disruption of the CWH41 gene leads to a K1 killer toxin-resistant phenotype and a 50% reduction in the cell wall beta 1,6-glucan level. CWH41 also displays strong genetic interactions with KRE1 and KRE6, two genes known ...
to be involved in the beta 1,6-glucan biosynthetic pathway. The cwh41 delta kre6 delta double mutant is nonviable; and the cwh41 delta kre1 delta double mutation results in strong synergistic defects, with a severely slow-growth phenotype, a 75% reduction in beta 1,6-glucan level, and the secretion of a cell wall glucomannoprotein, Cwp1p. These results provide strong genetic evidence indicating that Cwh41p plays a functional role, possibly as a new synthetic component, in the assembly of cell wall beta 1,6-glucan.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Blotting, Western, Cell Compartmentation, Cell Wall, Cloning, Molecular, Crosses, Genetic, Drug Resistance, Microbial, Endoplasmic Reticulum, Fungal Proteins, Genes, Fungal, Glucans, Glycoproteins, Killer Factors, Yeast, Membrane Glycoproteins, Membrane Proteins, Molecular Sequence Data, Mutation, Mycotoxins, Phenotype, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Analysis, DNA, alpha-Glucosidases, beta-Glucans
J. Bacteriol.
Date: Feb. 01, 1996
Download Curated Data For This Publication
15124
Switch View:
  • Interactions 2