A large set of estrogen receptor β-interacting proteins identified by tandem affinity purification in hormone-responsive human breast cancer cell nuclei.
Estrogen receptors α (ER-α) and β (ER-β) play distinct biological roles in onset and progression of hormone-responsive breast cancer, with ER-β exerting a modulatory activity on ER-α-mediated estrogen signaling and stimulation of cell proliferation by mechanisms still not fully understood. We stably expressed human ER-β fused to a tandem affinity ... purification-tag in estrogen-responsive MCF-7 cells and applied tandem affinity purification and nanoLC-MS/MS to identify the ER-β interactome of this cell type. Functional annotation by bioinformatics analyses of the 303 proteins that co-purify with ER-β from nuclear extracts identify several new molecular partners of this receptor subtype that represents nodal points of a large protein network controlling multiple processes and functions in breast cancer cells.
Mesh Terms:
Breast Neoplasms, Cell Line, Tumor, Cell Nucleus, Chromatography, Affinity, Estrogen Receptor beta, Estrogens, Female, Humans
Breast Neoplasms, Cell Line, Tumor, Cell Nucleus, Chromatography, Affinity, Estrogen Receptor beta, Estrogens, Female, Humans
Proteomics
Date: Jan. 01, 2011
PubMed ID: 21182203
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