Mak21p of Saccharomyces cerevisiae, a homolog of human CAATT-binding protein, is essential for 60 S ribosomal subunit biogenesis.
Mak21-1 mutants are unable to propagate M1 double-stranded RNA, a satellite of the L-A double-stranded RNA virus, encoding a secreted protein toxin lethal to yeast strains that do not carry M1. We cloned MAK21 using its map location and found that Mak21p is homologous to a human and mouse CAATT-binding ... protein and open reading frames in Schizosaccharomyces pombe and Caenorhabditis elegans. Although the human protein regulates Hsp70 production, Mak21p is essential for growth and necessary for 60 S ribosomal subunit biogenesis. mak21-1 mutants have decreased levels of L-A coat protein and L-A double-stranded RNA. Electroporation with reporter mRNAs shows that mak21-1 cells cannot optimally express mRNAs which, like L-A viral mRNA, lack 3'-poly(A) or 5'-cap structures but can normally express mRNA with both cap and poly(A). The virus propagation phenotype of mak21-1 is suppressed by ski2 or ski6 mutations, each of which derepresses translation of non-poly(A) mRNA.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA-Binding Proteins, Humans, Mice, Molecular Sequence Data, Neoplasm Proteins, Open Reading Frames, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Sequence Homology, Amino Acid, Transcription Factors
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA-Binding Proteins, Humans, Mice, Molecular Sequence Data, Neoplasm Proteins, Open Reading Frames, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Sequence Homology, Amino Acid, Transcription Factors
J. Biol. Chem.
Date: Oct. 30, 1998
PubMed ID: 9786894
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