Ito channels are octomeric complexes with four subunits of each Kv4.2 and K+ channel-interacting protein 2.
Mammalian voltage-gated K+ channels are assemblies of pore-forming alpha-subunits and modulating beta-subunits. To operate correctly, Kv4 alpha-subunits in the heart and central nervous system require recently identified beta-subunits of the neuronal calcium sensing protein family called K+ channel-interacting proteins (KChIPs). Here, Kv4.2.KChIP2 channels are purified, integrity of isolated complexes confirmed, ... molar ratio of the subunits determined, and subunit valence established. A complex has 4 subunits of each type, a stoichiometry expected for other channels employing neuronal calcium sensing beta-subunits.
Mesh Terms:
Animals, COS Cells, Calcium-Binding Proteins, Centrifugation, Density Gradient, Charybdotoxin, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Electrophysiology, Glycerol, Humans, Ions, Kv Channel-Interacting Proteins, Potassium Channels, Potassium Channels, Voltage-Gated, Protein Binding, Shal Potassium Channels, Time Factors, Xenopus laevis
Animals, COS Cells, Calcium-Binding Proteins, Centrifugation, Density Gradient, Charybdotoxin, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Electrophysiology, Glycerol, Humans, Ions, Kv Channel-Interacting Proteins, Potassium Channels, Potassium Channels, Voltage-Gated, Protein Binding, Shal Potassium Channels, Time Factors, Xenopus laevis
J. Biol. Chem.
Date: Feb. 13, 2004
PubMed ID: 14623880
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