Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins.
RNF43 is an oncogenic RING finger protein overexpressed in colorectal cancer. To dissect its biological functions, we explored RNF43-interacting proteins by pull-down assay and MS. We identified a heterodimer, p54nrb and PSF, as RNF43's binding partners and confirmed their physical interaction in vivo by the co-immunoprecipitation experiment. Immunofluorescence analysis revealed ... that co-expression of PSF relocates RNF43 from the nuclear periphery to the nucleoplasm. Thus, proteomic identification of RNF43-associated proteins sheds light on its dynamic interaction network in nuclear events.
Mesh Terms:
Active Transport, Cell Nucleus, Animals, COS Cells, Cell Line, Cell Nucleus, Cercopithecus aethiops, DNA-Binding Proteins, Dimerization, HeLa Cells, Humans, Nuclear Matrix-Associated Proteins, Octamer Transcription Factors, Oncogene Proteins, RNA-Binding Proteins
Active Transport, Cell Nucleus, Animals, COS Cells, Cell Line, Cell Nucleus, Cercopithecus aethiops, DNA-Binding Proteins, Dimerization, HeLa Cells, Humans, Nuclear Matrix-Associated Proteins, Octamer Transcription Factors, Oncogene Proteins, RNA-Binding Proteins
Proteomics
Date: Jul. 01, 2008
PubMed ID: 18655028
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