FCHo proteins are nucleators of clathrin-mediated endocytosis.

Clathrin-mediated endocytosis, the major pathway for ligand internalization into eukaryotic cells, is thought to be initiated by the clustering of clathrin and adaptors around receptors destined for internalization. However, here we report that the membrane-sculpting F-BAR domain-containing Fer/Cip4 homology domain-only proteins 1 and 2 (FCHo1/2) were required for plasma membrane ...
clathrin-coated vesicle (CCV) budding and marked sites of CCV formation. Changes in FCHo1/2 expression levels correlated directly with numbers of CCV budding events, ligand endocytosis, and synaptic vesicle marker recycling. FCHo1/2 proteins bound specifically to the plasma membrane and recruited the scaffold proteins eps15 and intersectin, which in turn engaged the adaptor complex AP2. The FCHo F-BAR membrane-bending activity was required, leading to the proposal that FCHo1/2 sculpt the initial bud site and recruit the clathrin machinery for CCV formation.
Mesh Terms:
Adaptor Protein Complex 2, Adaptor Proteins, Signal Transducing, Adaptor Proteins, Vesicular Transport, Animals, Calcium-Binding Proteins, Cell Line, Cell Membrane, Cells, Cultured, Clathrin, Clathrin-Coated Vesicles, Endocytosis, HeLa Cells, Humans, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Mice, Models, Molecular, Neurons, Phosphoproteins, Protein Multimerization, Protein Structure, Tertiary, Proteins, RNA Interference, Rats, Rats, Sprague-Dawley, Recombinant Fusion Proteins, Synaptic Vesicles
Science
Date: Jun. 04, 2010
Download Curated Data For This Publication
151437
Switch View:
  • Interactions 26