YNT20, a bypass suppressor of yme1 yme2, encodes a putative 3'-5' exonuclease localized in mitochondria of Saccharomyces cerevisiae.
Mutation of YME genes in yeast results in a high rate of mitochondrial DNA escape to the nucleus. The synthetic respiratory growth defect of yme1 yme2 yeast strains is suppressed by recessive mutations in YNT20. Inactivation of YNT20 creates a cold-sensitive respiratory growth defect that is more pronounced in a ... yme1 background and which is suppressed by yme2. Inactivation of YNT20 causes a qualitative reduction in the rate of mitochondrial DNA escape in yme1, but not yme2, strains, suggesting that YNT20 plays a role in the yme1-mediated mitochondrial DNA escape pathway. YNT20p is a soluble mitochondrial protein that belongs to a subfamily of putative 3'-5' exonucleases. Furthermore, conserved sequence elements in Yme2p suggest that this protein may also function as an exonuclease.
Mesh Terms:
ATP-Dependent Proteases, Adenosine Triphosphatases, Amino Acid Sequence, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA Primers, DNA, Mitochondrial, Exodeoxyribonuclease V, Exodeoxyribonucleases, Genes, Fungal, Mitochondria, Molecular Sequence Data, Mutation, Phenotype, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Suppression, Genetic
ATP-Dependent Proteases, Adenosine Triphosphatases, Amino Acid Sequence, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA Primers, DNA, Mitochondrial, Exodeoxyribonuclease V, Exodeoxyribonucleases, Genes, Fungal, Mitochondria, Molecular Sequence Data, Mutation, Phenotype, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Suppression, Genetic
Curr. Genet.
Date: Jan. 01, 1999
PubMed ID: 9933355
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