p75NTR signal transduction suppressed by BFAR and p75NTR interactions.

p75NTR is a low-affinity nerve growth factor receptor, which promotes cell proliferation as a positive modulator of high-affinity receptor TrkA, as well as binds with cell ligands to induce apoptosis and mediate death signals. To analyze the regulatory mechanisms of p75NTR, the present study utilized a new membrane yeast two-hybrid ...
system to screen a human fetal brain cDNA library. Results identified BFAR, a novel protein that interacts with p75NTR. Interaction specificity was verified by membrane yeast two-hybrid co-transformation assays, in vitro GST pull-down assays, and in vitro co-immunoprecipitation assays. The fluorescent subcellular localization assay revealed that the two proteins co-localized within the cytoplasm. BFAR overexpression in PC-12 and HEK293T cells inhibited the NFκB and JNK signaling pathway, as determined with the luciferase test. Co-transfected p75NTR and BFAR in HEK293T or PC-12 cells, respectively, increased the percentage of cells in the G2/M phase, decreased the number of S-phase cells, and did not change the number of G0/G1-phase cells.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Animals, Apoptosis Regulatory Proteins, Brain, Cell Cycle, Cytoplasm, Flow Cytometry, Gene Library, Green Fluorescent Proteins, HEK293 Cells, HeLa Cells, Humans, Immunoprecipitation, Membrane Proteins, Microscopy, Fluorescence, Nerve Tissue Proteins, PC12 Cells, Protein Binding, Rats, Receptor, Nerve Growth Factor, Receptors, Nerve Growth Factor, Signal Transduction, Transfection, Two-Hybrid System Techniques
Sci China Life Sci
Date: Apr. 01, 2012
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