A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress.

MAPKAP kinase-2 and MAPKAP kinase-3 were both activated in response to cellular stress, interleukin-1 and tumour necrosis factor in KB and HeLa cells, and with identical kinetics. Activation of MAPKAP kinase-3, like MAPKAP kinase-2, was prevented by SB 203580, a specific inhibitor of SAPK-2, the upstream activator of MAPKAP kinase-2. ...
MAPKAP kinase-3 and MAPKAP kinase-2 phosphorylated peptide substrates with similar kinetic constants and phosphorylated the same serine residues in HSP27 at the same relative rates. These results establish that MAPKAP kinase-3 lies 'downstream' of SAPK-2 and that it is likely to have overlapping or identical substrates to MAPKAP kinase-2 in vivo.
Mesh Terms:
Amino Acid Sequence, Animals, Antibody Formation, Cytokines, Enzyme Activation, Humans, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Phosphorylation, Precipitin Tests, Protein-Serine-Threonine Kinases, Rabbits, Stress, Physiological, Substrate Specificity
FEBS Lett.
Date: Sep. 02, 1996
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