Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry.
To understand the mechanisms by which CDKs regulate cell cycle progression, it is necessary to identify and characterize the physiological substrates of these kinases. We have developed a screening method to identify novel CDK substrates. One of the cDNAs identified in the screen is identical to the recently isolated NPAT ... gene. Here we show that NPAT associates with cyclin E-CDK2 in vivo and can be phosphorylated by this CDK. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry, and this effect is enhanced by coexpression of cyclin E-CDK2. These results suggest that NPAT is a substrate of cyclin E-CDK2 and plays a role in S-phase entry.
Mesh Terms:
CDC2-CDC28 Kinases, Cell Cycle Proteins, Cyclin E, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinases, G1 Phase, Gene Library, Humans, Nuclear Proteins, Protein Binding, Protein-Serine-Threonine Kinases, Proteins, Recombinant Proteins, S Phase, Selection, Genetic, Substrate Specificity
CDC2-CDC28 Kinases, Cell Cycle Proteins, Cyclin E, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinases, G1 Phase, Gene Library, Humans, Nuclear Proteins, Protein Binding, Protein-Serine-Threonine Kinases, Proteins, Recombinant Proteins, S Phase, Selection, Genetic, Substrate Specificity
Genes Dev.
Date: Feb. 15, 1998
PubMed ID: 9472014
View in: Pubmed Google Scholar
Download Curated Data For This Publication
152587
Switch View:
- Interactions 12