Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins.
Fission yeast Rhp23 and Pus1 represent two families of multiubiquitin chain-binding proteins that associate with the proteasome. We show that both proteins bind to different regions of the proteasome subunit Mts4. The binding site for Pus1 was mapped to a cluster of repetitive sequences also found in the proteasome subunit ... SpRpn2 and the anaphase-promoting complex/cyclosome (APC/C) subunit Cut4. The putative role of Pus1 as a factor involved in allocation of ubiquitinylated substrates for the proteasome is discussed.
Mesh Terms:
Amino Acid Sequence, Carrier Proteins, Cysteine Endopeptidases, DNA-Binding Proteins, Fungal Proteins, Hydro-Lyases, Ligases, Molecular Sequence Data, Multienzyme Complexes, Proteasome Endopeptidase Complex, Protein Binding, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment, Ubiquitin-Protein Ligase Complexes
Amino Acid Sequence, Carrier Proteins, Cysteine Endopeptidases, DNA-Binding Proteins, Fungal Proteins, Hydro-Lyases, Ligases, Molecular Sequence Data, Multienzyme Complexes, Proteasome Endopeptidase Complex, Protein Binding, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment, Ubiquitin-Protein Ligase Complexes
J. Biol. Chem.
Date: May. 09, 2003
PubMed ID: 12615927
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