Fission yeast cdc24(+) encodes a novel replication factor required for chromosome integrity.

Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 38232, USA. kathy.gould@mcmail.vanderbilt.edu
A mutation within the Schizosaccharomyces pombe cdc24(+) gene was identified previously in a screen for cell division cycle mutants and the cdc24(+) gene was determined to be essential for S phase in this yeast. We have isolated the cdc24(+) gene by complementation of a new temperature-sensitive allele of the gene, cdc24-G1. The DNA sequence predicts the presence of an open reading frame punctuated by six introns which encodes a pioneer protein of 58 kD. A cdc24 null mutant was generated by homologous recombination. Haploid cells lacking cdc24(+) are inviable, indicating that cdc24(+) is an essential gene. The transcript of cdc24(+) is present at constant levels throughout the cell cycle. Cells lacking cdc24(+) function show a checkpoint-dependent arrest with a 2N DNA content, indicating a block late in S phase. Arrest is accompanied by a rapid loss of viability and chromosome breakage. An S. pombe homolog of the replicative DNA helicase DNA2 of S. cerevisiae suppresses cdc24. These results suggest that Cdc24p plays a role in the progression of normal DNA replication and is required to maintain genomic integrity.
Mesh Terms:
Alleles, Amino Acid Sequence, Base Sequence, Cell Cycle, Cell Cycle Proteins, Cell Division, Fungal Proteins, Genetic Complementation Test, Genotype, Guanine Nucleotide Exchange Factors, Introns, Molecular Sequence Data, Proto-Oncogene Proteins, Recombination, Genetic, Repressor Proteins, Restriction Mapping, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Sequence Alignment, Sequence Homology, Amino Acid, Temperature
Genetics Jul. 01, 1998; 149(3);1221-33 [PUBMED:9649516]
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