A scanning peptide array approach uncovers association sites within the JNK/beta arrestin signalling complex.
Beta arrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/beta arrestin signalling complex. We show that beta arrestin 1 and beta arrestin 2 associate ... with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known 'common docking' motif. In the N-domain and C-terminus of beta arrestin 1 and beta arrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the beta arrestin 1 and beta arrestin 2 we identify a novel MKK4 docking site.
Mesh Terms:
Amino Acid Sequence, Animals, Arrestins, MAP Kinase Kinase 4, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase 10, Models, Molecular, Molecular Sequence Data, Peptide Library, Peptides, Protein Array Analysis, Protein Binding, Recombinant Fusion Proteins
Amino Acid Sequence, Animals, Arrestins, MAP Kinase Kinase 4, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase 10, Models, Molecular, Molecular Sequence Data, Peptide Library, Peptides, Protein Array Analysis, Protein Binding, Recombinant Fusion Proteins
FEBS Lett.
Date: Oct. 20, 2009
PubMed ID: 19782076
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