Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment.
Maintenance of chromosomal stability relies on coordination between various processes that are critical for proper chromosome segregation in mitosis. Here we show that monopolar spindle 1 (Mps1) kinase, which is essential for the mitotic checkpoint, also controls correction of improper chromosome attachments. We report that Borealin/DasraB, a member of the ... complex that regulates the Aurora B kinase, is directly phosphorylated by Mps1 on residues that are crucial for Aurora B activity and chromosome alignment. As a result, cells lacking Mps1 kinase activity fail to efficiently align chromosomes due to impaired Aurora B function at centromeres, leaving improper attachments uncorrected. Strikingly, Borealin/DasraB bearing phosphomimetic mutations restores Aurora B activity and alignment in Mps1-depleted cells. Mps1 thus coordinates attachment error correction and checkpoint signaling, two crucial responses to unproductive chromosome attachments.
Mesh Terms:
Alleles, Cell Cycle Proteins, Cell Line, Tumor, Chromosomes, Human, Enzyme Activation, HeLa Cells, Humans, Kinetochores, Microtubules, Mitotic Spindle Apparatus, Mutation, Phosphorylation, Plasmids, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, RNA, Small Interfering, Recombinant Proteins, Transfection
Alleles, Cell Cycle Proteins, Cell Line, Tumor, Chromosomes, Human, Enzyme Activation, HeLa Cells, Humans, Kinetochores, Microtubules, Mitotic Spindle Apparatus, Mutation, Phosphorylation, Plasmids, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, RNA, Small Interfering, Recombinant Proteins, Transfection
Cell
Date: Jan. 25, 2008
PubMed ID: 18243099
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